1. Set
up pH and conductivity probe.
2. Run pH calibration procedure.
3. Rinse 5 L test bucket:
a) Scrub out all visible foreign materials and rinse well with tap water.
b) Rinse with distilled water 3 times.
i) poor straight from distilled water tap
c) Rinse with ~50 mL of 0.05M HCl
d) Rinse with distilled water
i) use the rinse bottle, make sure the stream is hard and direct on all surfaces
4. Put ~1.5 L of distilled water into test bucket.
5. Weigh
out 1.1 mg of Eu nitrate in a weighing boat.
6. Tap the Eu off of the weighing boat into the distilled water, then dip
it into the water to make sure all Eu is off, stir well with glass stirring
rod.
7. Add more distilled water to the solution until you have 3 L, stir well
with glass stirring rod.
8. Clean
pH, Conductivity and Temperature Probes with distilled water.
9. Place probes into solution, start a stop watch, and stir
solution well with probes.
10. Monitor
probe read outs and record time, temp, pH and conductivity every 5 min.
11. Leave one person monitoring solution for every 5 min. for 30 min.
12. Wash Plant:
a) Rinse off all dirt and foreign substances with tap water.
i) do this by carefully separating each root and rinsing it individually
b) Rinse roots with distilled water
i) poor straight from distilled water tap
c) Place
in ~ 50mL 0.05M HCl for ~1 min.
d) Rinse with distilled water
i) use the rinse bottle, make sure the stream is hard and direct on all surfaces
e) Weigh plant
f) Allow plant to drip dry for ~ 5 min.
13. After sixth reading from solution (30 min) put plant into solution
and restart timer.
14. Stir
solution and plant in the upper right hand corner of the test bucket
with probes.
15. Continue
to take readings every 5 min for the next 4 hours, be sure to stir
probes before each reading.
16. After 4 hours stop trial and take
2 samples of the water using a disposable glass pipette, storing them in
a plastic test tube with top for future use.
17. Keep sample of roots in a test tube also
18. Clean
probes with distilled water.
19. Store pH probe in buffer, and conductivity probe dry.
20. Rinse all buckets used with tap water to get excess root bits out.
1. Set
up pH and conductivity probe.
2. Run pH calibration procedure.
3. Rinse 5 L test bucket:
a) Scrub out all visible foreign materials and rinse well with tap water.
b) Rinse with distilled water 3 times.
i) poor straight from distilled water tap
c) Rinse with ~50 mL of 0.05M HCl
d) Rinse with distilled water
i) use the rinse bottle, make sure the stream is hard and direct on all surfaces
4. Put ~1.5 L of distilled water into test bucket.
5. Weigh
out 1.1 mg of Eu nitrate in a weighing boat.
6. Tap the Eu off of the weighing boat into the distilled water, then dip
it into the water to make sure all Eu is off, stir well with glass stirring
rod.
7. Add more distilled water to the solution until you have 3 L, stir well
with glass stirring rod.
8. Clean
pH, Conductivity and Temperature Probes with distilled water.
9. Place probes into solution, start a stop watch, and stir
solution well with probes.
10. Monitor
probe read outs and record time, temp, pH and conductivity every 5 min.
11. After sixth reading from solution (30 min) put root bag into solution
and restart timer.
12. Hold root bag under the surface of the solution and spend 5-10 min.
beating the air bubbles out of the bag with two glass stir rods
a) there will be one big bubble that won't come out, be sure that when
you allow the bag to float up there are no roots in the part of the bag
that is above the surface.
b) the root bag will float on the top of the water just under the surface
13. Stir
solution and root bag in the upper right hand corner of the test bucket
with probes.
14. Continue
to take readings every 5 min for the next 4 hours, be sure to stir
probes before each reading.
15. After 4 hours stop trial and take
2 samples of the water using a disposable glass pipette, storing them in
a plastic test tube with top for future use.
16. Keep sample of root bag in a test tube also
17. Clean
probes with distilled water.
18. Store pH probe in buffer, and conductivity probe dry.
19. Rinse all buckets used with tap water to get excess root bits out.
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1. Using 31.4% by mass HCl weigh out 58.06 g of HCl into a beaker.
2. Fill a 1000mL volumetric flask about half full with distilled water.
3. Poor the 58.06 g of HCl into the flask and stir.
4. Rinse beaker that held HCl out with distilled water three times, each
time pouring the rinse into the solution.
a) this ensures that all of the HCl goes into the diluted solution
5. Fill volumetric flask up too 1000mL with distilled water and stir.
6. Put a rubber stopper in neck and invert flask 10 times.
7. Label flask with solution concentration ( 0.5M HCl), date, and initials.
8. Store with rubber stopper in place
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1. Poor
50mL of 0.5M HCl into a 50mL graduated cylinder
2. Poor 0.5M HCl into a 500mL flask
3. Rinse the graduated cylinder 3 times with distilled water, each time
pouring the rinse into the flask.
4. Fill
the flask up to 250mL with distilled water and stir.
5. Fill
the flask up to 500mL with distilled water and stir.
6. Put a rubber stopper into the neck of the flask and invert the flask
10 times.
7. Label flask with solution concentration ( 0.05M HCl), date, and initials.
8. Store with rubber stopper in place
Return to Live Plant Trial Procedure
Return to Dried Plant Trial Procedure
Return to Root Bag Preparation Procedure
1. Pre-heat drying oven too 70 degrees C
2. Wash Plant:
a) Rinse off all dirt and foreign substances with tap water.
i) do this by carefully separating each root and rinsing it individually
b) Rinse roots with distilled water
i) poor straight from distilled water tap
c) Place
in ~ 50mL 0.05M HCl for ~1 min.
d) Rinse with distilled water
i) use the rinse bottle, make sure the stream is hard and direct on all surfaces
e) Weigh plant
3. Break all roots off of plant and weigh fresh
root weight.
4. Place
roots on two sheets of tin foil.
5. Place
roots in drying oven.
6. Allow
roots to sit in the drying oven for 6 days, monitoring the temp.
a) the temp. of the drying oven will drop after plants are put in and will need to be turned up.
7. Take roots out of the drying oven.
8. Place a tin foil on a balence and "zero the scale out"
9. Carefully cut roots into 1/4-1/2 inch pieces with scissors over the
scales.
10. Record total mass, and divide by number of root bags desired.
a) this is the amount you would weigh out for each root bag
11. Weigh out the amount desired for one root bag on a piece of tin
foil.
12. Make bag
a) Stretch 1 leg of a pair of nylons out to 100cm.
b) Allow nylon to return to unstreched position and then repeat.
c) Cut two 30cm lengths from the leg
i) be sure to not include the toe or top of leg
d) Tie a tight not in one end of the bag
a) Poor weighed out amounts into the bag using a plastic funnel
b) Use a stir rod to push roots into bag.
c) Tie the open end of bag in a tight knot near the end.
14. Store finished root bags in clean tin foil until use to avoid contamination.
Return to Live Plant Trial Procedure
Return to Dried Plant Trial Procedure
Follow Live Plant Trial Procedure without putting Eu into solution (just monitor a plant in distilled water)
Follow Live Plant Trial Procedure without putting a plant into the solution (just monitor an Eu Solution)
Follow Dried Root Trial Procedure substituting an empty root bag for the filled one (just monitor an empty nylon bag in an Eu solution)
Return to Live Plant Trial Procedure
Return to Dried Plant Trial Procedure